醫(yī)學(xué)免費(fèi)論文:HPV16E6蛋白在宮頸病變組織中的表達(dá)及臨床應(yīng)用
【摘要】 目的 研究人乳頭瘤病毒16型E6蛋白(human papillomavirus16 E6 protein,HPV16E6)在宮頸病變組織中的表達(dá)及其與臨床病理特征之間的關(guān)系。方法 采用免疫組化SP法檢測(cè)HPV16E6蛋白在宮頸正常組織、慢性炎癥、宮頸上皮內(nèi)瘤變(cervical intraepithelial neoplasia,CIN)、浸潤(rùn)癌上皮中的陽(yáng)性表達(dá)情況。結(jié)果 HPV16E6在正常宮頸、宮頸炎、CIN,宮頸癌各組中,陽(yáng)性表達(dá)率分別為0.5%、38.35%、46.15%、71.11%,4組比較差異有統(tǒng)計(jì)學(xué)意義(χ2=27.50,P<0.01)。經(jīng)χ2分割后比較(α′=0.007143),宮頸癌組、CIN組、宮頸炎組的陽(yáng)性率顯著高于正常宮頸組(P<0.05),宮頸癌組的陽(yáng)性率顯著高于宮頸炎組(P<0.01)。HPV16E6與年齡、病理類型、宮旁或脈管浸潤(rùn)相關(guān)(P<0.05)。結(jié)論 HPV16E6與宮頸癌的啟動(dòng)、發(fā)生、發(fā)展及侵襲轉(zhuǎn)移均密切相關(guān)。HPV疫苗對(duì)于宮頸癌的防治有廣泛的應(yīng)用前景。
【關(guān)鍵詞】 人乳頭瘤病毒16型E6蛋白;免疫組織化學(xué);宮頸腫瘤;HPV疫苗
The expression and clinical significance of HPV16E6 in the pathological tissues of cervix ZHANG Lihua,XIN Huadong,WU Shiqian.Department of Gynecology, Handan Central Hospital,Hebei,Handan 056001,China
【Abstract】 Objective To investigate the relationship between the expression of human papillomavirus16 E6 protein (HPV16E6) and clinical pathological characteristics.Methods The expression of HPV16E6 protein in patients with cervical cancer, cervical intraepithelial neoplasia (CIN),chronic cervicitis and normal cervical epithelium were examined by immunohistochemitry.Results The positive rate of HPV16E6 protein was 71.11%,46.15%,38.35%,0.5% in cervical cancer,CIN,chronic cervicitis and normal cervical epithelium respectively. There were significant differences in the positive rates among the 4 groups (P<0.01).The positive rate in cervical cancer group was significantly higher than that in chronic cervicitis group and normal cervical epithelium group(P<0.01).The positive rate in CIN and cervicitis group was significantly higher than that normal cervical epithelium group(P<0.01).The positive expression of HPV16-E6 was correlated with the age of the patients ,pathological types and the infiltration of latero-uterus or haemal tube(P<0.05).Conclusion HPV16E6 plays an important role in the genesis,development,invasion and metastasis of cervical carcinoma. The vaccine of HPV has wide application prospect in the prevention and treatment of cervical carcinoma醫(yī).學(xué).全.在.線52667788.cn.
【Key words】 HPV16E6;immunohistochemitry;cervical cancer;vaccine of HPV
宮頸癌是女性最常見(jiàn)的惡性腫瘤[1],在我國(guó)發(fā)病率居女性惡性腫瘤的首位,是導(dǎo)致女性死亡的主要原因[2]。高危型HPV的持續(xù)感染是宮頸癌及其癌前病變的直接病因,沒(méi)有持續(xù)高危HPV感染,便沒(méi)有可能發(fā)生宮頸鱗癌[3,4]。本研究采用免疫組化法檢測(cè)人不同宮頸病變組織中的HPV16E6蛋白的陽(yáng)性率,探討HPV16E6蛋白不同宮頸病變組織中的表達(dá)情況及其與宮頸癌臨床病理參數(shù)之間的關(guān)系。
1 資料與方法
1.1 一般資料 本研究標(biāo)本取自2007年3月至2009年3月在河北省邯鄲市中心醫(yī)院婦科初診的宮頸癌患者45例。年齡26~61歲,平均年齡46歲。其中鱗癌36例,腺癌6例,腺鱗癌及透明細(xì)胞癌3例。根據(jù)國(guó)際婦產(chǎn)科聯(lián)盟(FIGO,2000年)標(biāo)準(zhǔn)分期:Ⅰ期25例,Ⅱ期17例,Ⅲ期3例。組織分化程度G1、G2、G3分別為12例、20例、13例。患者術(shù)前均未接受放、化療。選擇同期因婦科良性疾病手術(shù)治療或門診治療患者的正常宮頸組織21例,宮頸慢性炎癥73例,CIN 26例。所有標(biāo)本于離體0.5 h內(nèi)取材,以10%中性甲醛固定,石蠟包埋,切片備用。所有取材標(biāo)本均常規(guī)HE染片確定病理學(xué)診斷、組織學(xué)類型和分級(jí),病歷資料完整。