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醫(yī)學(xué)免費論文:肺炎支原體肺炎患兒血清細胞因子與免疫球蛋白的檢測及其臨床意義
【摘要】 目的 探討檢測肺炎支原體肺炎(MPP)患兒血清細胞因子(IL6、IL8、TNFα)、免疫球蛋白(IgA、IgM、IgG)及補體(C3、C4)的臨床意義。方法 60例MPP患兒(觀察組)和60例健康兒童(正常對照組)為研究對象，采用放射免疫分析法測定IL6、IL12、TNFα，采用速率散射比濁法測定血清免疫球蛋白(Ig)和補體(C)。結(jié)果 觀察組血清IL6、IL8的水平與正常對照組比較上升(P<0.05);而TNFα水平2組比較差異無統(tǒng)計學(xué)意義(P>0.05);觀察組血清免疫球蛋白IgA、IgM、IgG水平、補體C水平與正常對照組比較上升(P<0.05)。結(jié)論 血清IL6、IL8、免疫球蛋白IgA、IgM、IgG，補體C3、4在MPP中起著重要作用，檢測這些指標(biāo)可以較準(zhǔn)確地判定MMP患兒病情和預(yù)后，值得臨床推廣應(yīng)用。
【關(guān)鍵詞】 肺炎支原體肺炎;白介素6;白介素8;腫瘤壞死因子;免疫球蛋白Ig;補體C
Clinical significance of serum cytokines and immunoglobulin levels in children with mycoplasma pneumoniae pneumonia XIE Zhicai, LV Hui, ZHOU Shuming. Longgang Central Hospital, Guangdong, Shenzheng 518116,China
【Abstract】 Objective To investigate the clinical significance of detecting serum cytokines (IL6, IL8 and TNFα), immunoglobulins (IgA, IgM and IgG) and complements (C3 and C4) levels in children with mycoplasma pneumoniae pneumonia (MPP). Methods 60 children with MPP (observation group) and 60 healthy children (control group) were enrolled in the study. The serum levels of IL6, IL8 and TNFα were detected by ELISA method, the serum levels of immunoglobulins (IgA, IgM and IgG) and complements (C3 and C4) were determined by rate nephelometry method.Results The serum levels of IL6 and IL8 in observation group were significantly higher than those of control group (P<0.05),however there was no significant difference in TNFα levels between the two groups (P> 0.05). The serum levels of IgA, IgM，IgG，C3 and C4 in observation group were significantly higher than those of control group (P<0.05).Conclusion The serum levels of IL6 and IL8, immunoglobulins (IgA, IgM and IgG) and complements (C3 and C4) play an important role in MPP.The detection of these parameters can more accurately determine the disease condition and patients’prognosis,which is worthy to be popularized.
【Key words】 mycoplasma pneumoniae pneumonia;IL6; IL8; TNF;immunoglobulin; complement
肺炎支原體肺炎(MPP)又可稱原發(fā)性非典型性肺炎，其病原體為肺炎支原體(MP)。MP廣泛分布于自然界，是一種介于細菌和病毒之間的、能獨立生活的最小微生物，是小兒呼吸道感染中較常見的病原體，是引起小兒下呼吸道感染的重要病原之一。已有研究認為，在MPP發(fā)病機制中，眾多細胞因子都直接或間接地參與了免疫反應(yīng)、炎性反應(yīng)及免疫損傷[1]。認為內(nèi)源性炎性介質(zhì)白介素6(IL6)、IL8和腫瘤壞死因子α(TNFα)的大量釋放，是引起繼發(fā)性肺損傷的重要因素之一[2]。在MPP體液免疫方面，免疫球蛋白IgA、IgM、IgG、C3、C4與疾病的嚴重程度和病程長短也是目前人們研究的焦點。因此本文對MPP患兒血清炎性細胞因子IL6、IL12、TNFα和免疫球蛋白IgA、IgM、IgG、C3、C4水平進行研究，以探討其在診治小兒MPP中的價值，并提供理論依據(jù)醫(yī).學(xué)全.在.線網(wǎng)站52667788.cn。
1 資料與方法
