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醫(yī)學(xué)免費(fèi)論文:高頻左心房起搏制作兔慢性房顫模型及電生理特性研究
【摘要】 建立兔左心房高頻起搏心房顫動(dòng)(AF)動(dòng)物模型，并進(jìn)行電生理特性研究。 【方法】 16只健康新西蘭兔隨機(jī)分為：對(duì)照組和起搏組兩組。兩組均在左心房外膜縫合固定一起搏電極，起搏組以1 000 min-1的頻率進(jìn)行高頻起搏;對(duì)照組不起搏。采用心外膜程序起搏技術(shù)測(cè)定心房肌的電生理特性。 【結(jié)果】 ①起搏組3周后房顫誘發(fā)率87.5%，與對(duì)照組比較統(tǒng)計(jì)學(xué)有差異(P < 0.01)，其中持續(xù)性AF 3/8例;②起搏組經(jīng)心外膜程序起搏，在高頻刺激1 h、1周、3周后心房有效不應(yīng)期(ERPA)均縮短，與對(duì)照組比較有顯著統(tǒng)計(jì)學(xué)差異(P < 0.01);③頻率適應(yīng)性方面，起搏組表現(xiàn)為頻率適應(yīng)不良，在3周時(shí)最明顯，與對(duì)照組比有統(tǒng)計(jì)學(xué)意義(P < 0.05);④起搏組3周后P波時(shí)限比對(duì)照組延長(zhǎng)，但兩者比較無統(tǒng)計(jì)學(xué)差別(P﹥0.05);⑤起搏組1 h至3周，ERPA縮短、相對(duì)不應(yīng)期(RRPA)延長(zhǎng)，與對(duì)照組比較統(tǒng)計(jì)學(xué)有差異(P < 0.01)、早搏刺激的心房間傳導(dǎo)時(shí)間(IACD)延長(zhǎng)，與對(duì)照組比較統(tǒng)計(jì)學(xué)上無差別(P﹥0.05)�！窘Y(jié)論】 兔左心房高頻起搏能有效建立慢性AF動(dòng)物模型;主要電生理改變有：ERPA縮短、頻率適應(yīng)不良、P波時(shí)限延長(zhǎng)。
【關(guān)鍵詞】 心房刺激; 心房顫動(dòng); 動(dòng)物模型; 心房電生理特性醫(yī).學(xué).全.在.線52667788.cn
Induction of Atrial Fibrillation by Left Atrial High-Frequency Pacing and Investigationof Electrophysiological Characteristics of Atrial in RabbitsLIU Li-juan， ZHENG Dong-dan， LI Shu-bing， PAN De-mao， WANG Qiang， ZHANG Wei1， GAO Xiu-ren1. Department of Cardiology; 2. Department of Emergency，Huangpu Hospital， The First Affiliated Hospitalof Sun Yat-Sen University， Guangzhou 510700， ChinaAbstract： 【Objective】 To establish the model of atrial fibrillation (AF) by high-frequency pacing and explore the atrial electrophysiological characters in rabbits. 【Methods】 Sixteen rabbits were randomly divided into pacing group and control group. A pacing electricity pole was fixed under the adventitia of left atrium in all experiment animals. The pacing group was stimulated in a frequency of 1 000 bpm and the control group underwent without pacing stimulation. The technique of programmed stimulating was used to measure electrophysiological indexes of atrial in the groups. 【Results】 ①87.5% of the animals in pacing group suffered from AF under 3 weeks high frequency pacing， which was significantly higher than that of control group (P < 0.01). Permanent AF was found in 3 out of 8 cases. ②Atrial effective refractory period (ERPA) was statistically shortened in the pacing group comparing to the control group after 1 hour， 1 week and 3 weeks stimulating (P < 0.01， respectively). ③The pacing group showed a significantly poor performance of frequency adaptability comparing to the control group after 3 weeks stimulating (P < 0.05). ④The P-wave interval was longer in pacing group than that of the control group after 3 weeks pacing， but no significantly different was found (P > 0.05). ⑤ERPA was obviously shortened and RRPA was significantly prolonged in the pacing group after 1 hour to 3 weeks stimulating (P < 0.01)， but there was no difference significantly in inter-atrial conduction defect (IACD) between the two groups (P﹥0.05). 【Conclusions】 The animal model of chronic atrial fibrillation could be successfully established by high frequency pacing of atrial in rabbits. The apparent electrophysiological changes of this model include： the frequency inadaptability， shortening of ERPA and extension of P-wave interval.
Key words： artrial stimulation; atrial fibrillation; animal model; artrial electrophysiological charactoristics
2 結(jié) 果
