公開(公告)號 | CN1196712C |
公開(公告)日 | 2005.04.13 |
申請(專利)號 | CN01106757.8 |
申請日期 | 2001.02.21 |
專利名稱 | 一種類人膠原蛋白及其生產(chǎn)方法 |
主分類號 | C07K14/78 |
分類號 | C07K14/78;C12P21/02 |
分案原申請?zhí)? | |
優(yōu)先權(quán) | |
申請(專利權(quán))人 | 范代娣 |
發(fā)明(設(shè)計(jì))人 | 范代娣 |
地址 | 710075 陜西省西安市西北大學(xué)化工系 |
頒證日 | |
國際申請 | |
進(jìn)入國家日期 | |
專利代理機(jī)構(gòu) | 西安新思維專利事務(wù)所有限公司 |
代理人 | 李罡 |
國省代碼 | 陜西;61 |
主權(quán)項(xiàng) | 一種類人膠原蛋白,其特征在于:其序列如下HDPVVLQRRDWENPGVTQLNRHLAHAHPPFASDHPMGAPGPAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAHGPAGALGAHGPAGPLGPAGPPGSAGAPGAHGPAGPLGAHGPAGPLGAHGPAGPLGAHGPAGPLGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAHGPAGPLGAHGPAGPLGAHGPAGPLGAHGPAGPLGAPGPAGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAHGPAGPLGAHGPAGPLGAHGPAGPLGAHGPAGPLGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAPGPAGPPGSAGAPGPPGAHGPAGPLGAHGPAGPLGAHGPAGPLGAMGAPGATGLSAGATHGLVTCGL,所述結(jié)構(gòu)為三鏈、三螺旋結(jié)構(gòu),其核酸長度為1071bp。 |
摘要 | 本發(fā)明公開一種類膠原蛋白及通過基因工程菌生產(chǎn)類膠原蛋白的方法。其類人膠原蛋白的生產(chǎn)方法包括:類人膠原蛋白的工程菌的構(gòu)建;將工程菌的發(fā)酵培養(yǎng);再經(jīng)類人膠原蛋白的誘導(dǎo)及表達(dá),提純,獲得目的蛋白。該方法獲得的重組蛋白在單細(xì)胞內(nèi)的表達(dá)高,其類膠原蛋白為螺旋結(jié)構(gòu),異于動(dòng)物體原蛋白的交叉結(jié)構(gòu),因此它具有很好的可加工性而不改變其分子量,因此類人膠原蛋白可加工成手術(shù)縫合線、人工皮膚、膠片余層,人工器官涂層等,也可與鹵化銀、染料等結(jié)合形成具有優(yōu)良表面粘性的涂料。 |
國際公布 |